Anti-EPAS1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA1631-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of EPAS1 using anti-EPAS1 antibody (BA1631-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPAS1 antigen affinity purified polyclonal antibody (BA1631-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EPAS1 at approximately 96 kDa. The expected band size for EPAS1 is at 96 kDa.
all(3)
Western blot analysis of EPAS1 using anti-EPAS1 antibody (BA1631-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse lun tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPAS1 antigen affinity purified polyclonal antibody (BA1631-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EPAS1 at approximately 96 kDa. The expected band size for EPAS1 is at 96 kDa.
all(3)
Western blot analysis of EPAS1 using anti-EPAS1 antibody (BA1631-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat RH35 whole cell lysates,
Lane 3: rat small intestine tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPAS1 antigen affinity purified polyclonal antibody (BA1631-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EPAS1 at approximately 96 kDa. The expected band size for EPAS1 is at 96 kDa.
all(3)

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产品简介>

产品名称

Anti-EPAS1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

bHLHe73; ECYT4; EPAS 1; EPAS1; HIF 1 alpha like factor; HIF 2 alpha; HIF2 alpha; HIF2A; HIF-2A; HIF2α; HIF-2α; HLF; Member of PAS protein 2; MOP2; PASD2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

EPAS1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha, identical to the related mouse and rat sequence.

计算分子量

96 kDa

实际分子量

96 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88% identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48% sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.

Uniprot ID

Q99814

文献引用格式

EPAS1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1631-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

endothelial PAS domain protein 1

蛋白名全称

Endothelial PAS domain-containing protein 1(EPAS-1)

实验方案>

实验步骤

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