Anti-EPAS1 Antibody (Clone#OTI2G5)

50μl/1280 100μl/2180 150μl/2800

bHLHe73; ECYT4; EPAS 1; EPAS1; HIF 1 alpha like factor; HIF 2 alpha; HIF2 alpha; HIF2A; HIF-2A; HIF2α; HIF-2α; HLF; Member of PAS protein 2; MOP2; PASD2

Monoclonal

human, mouse, rat

WB

EPAS1

OTI2G5

Mouse

IgG1

Human recombinant protein fragment corresponding to amino acids 584-870 of human EPAS1 (NP_001421) produced in E.coli.

96 kDa

96 kDa

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

500 ug/ml

Liquid

Stable for 12 months from date of receipt. Store at -20°C as received.

HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88% identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48% sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.

Q99814  

EPAS1 Antibody (Clone#OTI2G5) (Boster Biological Technology, Wuhan, China. Catalog#M01248-3)

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

endothelial PAS domain protein 1

Endothelial PAS domain-containing protein 1

Western blot (WB):

1:2000