Anti-SPHK1 Antibody

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of SPHK1 using anti-SPHK1 antibody (BA2865). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: RAJI whole cell lysates,
  Lane 2: MCF-7 whole cell lysates,
  Lane 3: Hut whole cell lysates,
  Lane 4: HELA whole cell lysates,
  Lane 5: 293T whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SPHK1 antigen affinity purified polyclonal antibody (BA2865) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SPHK1 at approximately 45-50 kDa. The expected band size for SPHK1 is at 43 kDa.

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  IHC analysis of SPHK1 using anti-SPHK1 antibody (BA2865).
  SPHK1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-SPHK1 Antibody (BA2865) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  ICC analysis of SPHK1 using anti- SPHK1 antibody (BA2865).
  SPHK1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SPHK1 Antibody (BA2865) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of U87 cells using anti-SPHK1 antibody (BA2865).
  Overlay histogram showing U87 cells stained with BA2865 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPHK1 Antibody (BA2865, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  Flow Cytometry analysis of U2OS cells using anti-SPHK1 antibody (BA2865).
  Overlay histogram showing U2OS cells stained with BA2865 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPHK1 Antibody (BA2865, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Western blot analysis of SPHK1 using anti-SPHK1 antibody (BA2865). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: RAJI whole cell lysates,
  Lane 2: MCF-7 whole cell lysates,
  Lane 3: Hut whole cell lysates,
  Lane 4: HELA whole cell lysates,
  Lane 5: 293T whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SPHK1 antigen affinity purified polyclonal antibody (BA2865) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SPHK1 at approximately 45-50 kDa. The expected band size for SPHK1 is at 43 kDa.

• 

  IHC analysis of SPHK1 using anti-SPHK1 antibody (BA2865).
  SPHK1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-SPHK1 Antibody (BA2865) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

• 

  ICC analysis of SPHK1 using anti- SPHK1 antibody (BA2865).
  SPHK1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SPHK1 Antibody (BA2865) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of U87 cells using anti-SPHK1 antibody (BA2865).
  Overlay histogram showing U87 cells stained with BA2865 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPHK1 Antibody (BA2865, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Flow Cytometry analysis of U2OS cells using anti-SPHK1 antibody (BA2865).
  Overlay histogram showing U2OS cells stained with BA2865 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPHK1 Antibody (BA2865, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.