Anti-SPHK1 Antibody (Clone#OTI1A6)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF

M01390-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-SPHK1 antibody (M01390-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-SPHK1 antigen affinity purified monoclonal antibody (M01390-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SPHK1 at approximately 42 kDa. The expected band size for SPHK1 is at 42 kDa.
all(5)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SPHK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M01390-1)
all(5)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPHK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M01390-1)
all(5)
Immunofluorescent staining of HeLa cells using anti-SPHK1 mouse monoclonal antibody.
all(5)
Anti-SPHK1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPHK1 .
all(5)

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产品简介>

产品名称

Anti-SPHK1 Antibody (Clone#OTI1A6)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

SK 1; sphingosine kinase 1; SPHK; SPHK1; SPHK1 phopho; SPK; SPK 1

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF

基因名称

SPHK1

克隆号

OTI1A6

宿主

Mouse

抗体亚型

IgG2a

免疫原

Full length human recombinant protein of human SPHK1(NP_068807) produced in HEK293T cell.

计算分子量

43 kDa

实际分子量

43.8 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot ID

Q9NYA1

文献引用格式

SPHK1 Antibody (Clone#OTI1A6) (Boster Biological Technology, Wuhan, China. Catalog#M01390-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

sphingosine kinase 1

蛋白名全称

Sphingosine kinase 1

实验方案>

实验步骤

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