Anti-MDM2 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB

BA3612-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of MDM2 using anti-MDM2 antibody (BA3612-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MDM2 antigen affinity purified polyclonal antibody (BA3612-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MDM2 at approximately 90-100 kDa. The expected band size for MDM2 is at 55 kDa.
all(1)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-MDM2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Double minute 2 protein; hdm2; HDMX; MDM2; Oncoprotein Mdm2; p53 binding protein Mdm2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

MDM2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human MDM2, different from the related mouse sequence by three amino acids, and different from rat sequence by two amino acids.

计算分子量

55 kDa

实际分子量

90-100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

Uniprot ID

Q00987

文献引用格式

MDM2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3612-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Mdm2, p53 E3 ubiquitin protein ligase homolog(mouse)

蛋白名全称

E3 ubiquitin-protein ligase Mdm2

实验方案>

实验步骤

引用文献(0)>

清除