Anti-MDM2 (Phospho-S166) Antibody (Clone#OTI4B3)

Mouse monoclonal antibody

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M00054-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM2.
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产品名称

Anti-MDM2 (Phospho-S166) Antibody (Clone#OTI4B3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Double minute 2 protein; hdm2; HDMX; MDM2; Oncoprotein Mdm2; p53 binding protein Mdm2

产品类型

Monoclonal

检验物种

human

应用范围

基因名称

MDM2

克隆号

OTI4B3

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 119-438 of human MDM2 (NP_002383)produced in E.coli.

计算分子量

55 kDa

实际分子量

55.8 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

Uniprot ID

Q00987

文献引用格式

MDM2 (Phospho-S166) Antibody (Clone#OTI4B3) (Boster Biological Technology, Wuhan, China. Catalog#M00054-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Mdm2, p53 E3 ubiquitin protein ligase homolog(mouse)

蛋白名全称

E3 ubiquitin-protein ligase Mdm2

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实验步骤

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