Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of THBS2 using anti-THBS2 antibody (BA3616-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-THBS2 antigen affinity purified polyclonal antibody (BA3616-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for THBS2 at approximately 160 kDa. The expected band size for THBS2 is at 130 kDa.
Figure 1. Western blot analysis of THBS2 using anti-THBS2 antibody (BA3616-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-THBS2 antigen affinity purified polyclonal antibody (BA3616-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for THBS2 at approximately 160 kDa. The expected band size for THBS2 is at 130 kDa.