Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (BA3639).
Lane 1: recombinant Human CD68 Protein 10ng,
Lane 2: recombinant Human CD68 Protein 5ng,
Lane 3: recombinant Human CD68 Protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (BA3639) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD68 at approximately 42.5 kDa.
Figure 2. IHC analysis of CD68 using anti-CD68 antibody (BA3639).
CD68 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3639) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (BA3639).
Lane 1: recombinant Human CD68 Protein 10ng,
Lane 2: recombinant Human CD68 Protein 5ng,
Lane 3: recombinant Human CD68 Protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (BA3639) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD68 at approximately 42.5 kDa.
Figure 2. IHC analysis of CD68 using anti-CD68 antibody (BA3639).
CD68 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3639) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.