Immunohistochemistry (IHC): | 1:50-400 |
Immunofluorescence (IF): | 1:50-400 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. IHC analysis of CD68 using anti-CD68 antibody (A00602-1) .
CD68 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (A00602-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 2. IF analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (A00602-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 1. IHC analysis of CD68 using anti-CD68 antibody (A00602-1) .
CD68 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (A00602-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 2. IF analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (A00602-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).