Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of anti-XAF1 antibody (BA4986). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: Rat RH35 whole cell lysates,Lane 3: Mouse HepA whole cell lysates.Use rabbit anti- XAF1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for XAF1 at approximately 34KD. The expected band size for XAF1 is at 34KD.
Figure 2.IHC analysis using anti-XAF1 antibody (BA4986). detected in paraffin-embedded section of human endometrialcancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.