Anti-XAF1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC

BA4986

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Figure 1. Western blot analysis of XAF1 using anti-XAF1 antibody (BA4986). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: Rat RH35 whole cell lysates,
Lane 3: Mouse HepA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-XAF1 antigen affinity purified polyclonal antibody (BA4986) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for XAF1 at approximately 34 kDa. The expected band size for XAF1 is at 35 kDa.
all(2)
Figure 2. IHC analysis of XAF1 using anti-XAF1 antibody (BA4986).
XAF1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-XAF1 Antibody (BA4986) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-XAF1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

XAF1; BIRC4BP; XIAPAF1; BIRC4-binding protein

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

XAF1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human XAF1.

计算分子量

35 kDa

实际分子量

34 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

XIAP associated factor-1, also known as XAF1, is a human gene. X-linked inhibitor of apoptosis(XIAP; MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP(BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. And alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

Uniprot ID

Q6GPH4

文献引用格式

XAF1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA4986)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

XIAP associated factor 1

蛋白名全称

XIAP-associated factor 1

实验方案>

实验步骤

引用文献(0)>

清除