Anti-XAF1 Antibody

筛选器： All WB ICC/IF FCM

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  Figure 1. Western blot analysis of XAF1 using anti-XAF1 antibody (PA1218). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Jurkat whole cell lysates.
  \After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-XAF1 antigen affinity purified polyclonal antibody (PA1218) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for XAF1 at approximately 35 kDa. The expected band size for XAF1 is at 35 kDa.

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  Figure 2. IF analysis of XAF1 using anti-XAF1 antibody (PA1218).
  XAF1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-XAF1 Antibody (PA1218) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.

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  Figure 3. Flow Cytometry analysis of SiHa cells using anti-XAF1 antibody (PA1218).
  Overlay histogram showing SiHa cells stained with PA1218 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XAF1 Antibody (PA1218) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Figure 1. Western blot analysis of XAF1 using anti-XAF1 antibody (PA1218). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Jurkat whole cell lysates.
  \After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-XAF1 antigen affinity purified polyclonal antibody (PA1218) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for XAF1 at approximately 35 kDa. The expected band size for XAF1 is at 35 kDa.

• 

  Figure 2. IF analysis of XAF1 using anti-XAF1 antibody (PA1218).
  XAF1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-XAF1 Antibody (PA1218) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.

• 

  Figure 3. Flow Cytometry analysis of SiHa cells using anti-XAF1 antibody (PA1218).
  Overlay histogram showing SiHa cells stained with PA1218 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XAF1 Antibody (PA1218) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.