Anti-NF-H/NF200/NEFH Antibody (Clone#N52)

Mouse monoclonal antibody

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筛选器： All WB IHC

BM0100

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- NEFH antibody (BM0100). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
Use mouse anti- NEFH 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for NEFH at approximately 200KD. The expected band size for NEFH is at 112KD.
all(3)
IHC analysis using anti- NEFH antibody (BM0100). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(3)
IHC analysis using anti- NEFH antibody (BM0100). detected in paraffin-embedded section of human gliom tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(3)

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产品简介>

产品名称

Anti-NF-H/NF200/NEFH Antibody (Clone#N52)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NEFH; NEH; NF; NF H; NF200; NF-H; Neurofilament H

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

NEFH

克隆号

N52

宿主

Mouse

抗体亚型

IgG1

免疫原

C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

计算分子量

112 kDa

实际分子量

117-220 kDa

成分

200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.

纯化方式

Ascites

浓度

200ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM), and 200 kD(NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein(NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions(exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease(CMT).

Uniprot ID

P12036

RRID

AB_2889947

文献引用格式

NF-H/NF200/NEFH Antibody (Clone#N52) (Boster Biological Technology, Wuhan, China. Catalog#BM0100)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

neurofilament, heavy polypeptide

蛋白名全称

Neurofilament heavy polypeptide

实验方案>

实验步骤

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