Anti-NF-H/NF200/NEFH Antibody (Clone#OTI10A7)

Mouse monoclonal antibody

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筛选器： All IHC WB

M05307-8

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-NEFH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M05307-8) (1:2000)
all(2)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFH (Cat# M05307-8).
all(2)

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产品简介>

产品名称

Anti-NF-H/NF200/NEFH Antibody (Clone#OTI10A7)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NEFH; NEH; NF; NF H; NF200; NF-H; Neurofilament H

产品类型

Monoclonal

检验物种

human, rat

应用范围

IHC, WB

基因名称

NEFH

克隆号

OTI10A7

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 778-1020 of human NEFH (NP_066554) produced in E.coli.

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Uniprot ID

P19246

文献引用格式

NF-H/NF200/NEFH Antibody (Clone#OTI10A7) (Boster Biological Technology, Wuhan, China. Catalog#M05307-8)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

neurofilament, heavy polypeptide

蛋白名全称

Neurofilament heavy polypeptide

实验方案>

实验步骤

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