| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |

Western blot analysis of anti- CDK4 Antibody (BM1572). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: PC-12 whole cell lysates.
Lane 4: NIH/3T3 whole cell lysates,
Lane 5: RAW264.7 whole cell lysates.
Use mouse anti- CDK4 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CDK4 at approximately 34KD. The expected band size for CDK4 is at 34KD.

Western blot analysis of anti- CDK4 Antibody (BM1572). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: PC-12 whole cell lysates.
Lane 4: NIH/3T3 whole cell lysates,
Lane 5: RAW264.7 whole cell lysates.
Use mouse anti- CDK4 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CDK4 at approximately 34KD. The expected band size for CDK4 is at 34KD.


