Anti-CDK4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF

PB9535

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CDK4 using anti-CDK4 antibody (PB9535). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: PC-12 whole cell lysates,
Lane 4: NIH/3T3 whole cell lysates,
Lane 5: RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDK4 antigen affinity purified polyclonal antibody (PB9535) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.
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IHC analysis of CDK4 using anti-CDK4 antibody (PB9535).
CDK4 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDK4 Antibody (PB9535) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of CDK4 using anti-CDK4 antibody (PB9535).
CDK4 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDK4 Antibody (PB9535) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of CDK4 using anti-CDK4 antibody (PB9535).
CDK4 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDK4 Antibody (PB9535) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IF analysis of CDK4 using anti-CDK4 antibody (PB9535).
CDK4 was detected in a paraffin-embedded section of human colon cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(5)

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产品简介>

产品名称

Anti-CDK4 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CDK4; Cell division protein kinase 4; CMM3; cyclin dependent kinase 4; PSK J3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF

基因名称

CDK4

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Cdk4 recombinant protein (Position: G201-E303). Human Cdk4 shares 94.2% amino acid (aa) sequence identity with both mouse and rat Cdk4.

计算分子量

34 kDa

实际分子量

34 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. And CDK4 expression and activity are required for cytokine responsiveness in T cells.

Uniprot ID

P11802

RRID

AB_3082153

文献引用格式

CDK4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9535)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin-dependent kinase 4

蛋白名全称

Cyclin-dependent kinase 4

实验方案>

实验步骤

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