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Anti-CDK4 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC IF

PB9535

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of CDK4 using anti-CDK4 antibody (PB9535). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: HELA whole cell lysates,
    Lane 2: K562 whole cell lysates,
    Lane 3: PC-12 whole cell lysates,
    Lane 4: NIH/3T3 whole cell lysates,
    Lane 5: RAW264.7 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDK4 antigen affinity purified polyclonal antibody (PB9535) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.

    all(5)
  • IHC analysis of CDK4 using anti-CDK4 antibody (PB9535).
    CDK4 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDK4 Antibody (PB9535) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of CDK4 using anti-CDK4 antibody (PB9535).
    CDK4 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDK4 Antibody (PB9535) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of CDK4 using anti-CDK4 antibody (PB9535).
    CDK4 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDK4 Antibody (PB9535) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IF analysis of CDK4 using anti-CDK4 antibody (PB9535).
    CDK4 was detected in a paraffin-embedded section of human colon cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(5)

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产品简介>

产品名称
Anti-CDK4 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CDK4; Cell division protein kinase 4; CMM3; cyclin dependent kinase 4; PSK J3
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, IF
基因名称
CDK4
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human Cdk4 recombinant protein (Position: G201-E303). Human Cdk4 shares 94.2% amino acid (aa) sequence identity with both mouse and rat Cdk4.
计算分子量
34 kDa
实际分子量
34 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. And CDK4 expression and activity are required for cytokine responsiveness in T cells.
Uniprot ID
P11802  
RRID
文献引用格式
CDK4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9535)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
cyclin-dependent kinase 4
蛋白名全称
Cyclin-dependent kinase 4
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunofluorescence (IF):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-CDK4 Antibody

    筛选器: All WB IHC IF

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