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Anti-Alpha Tubulin/TUBA1B Antibody (Clone#CG-20)

Rabbit Monoclonal Antibody

说明书

筛选器: All WB IHC

BM3885

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Figure 1. Western blot analysis of anti-Alpha Tubulin/TUBA1B antibody (BM3885). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human A549 whole cell lysates,
    Lane 3: human K562 whole cell lysates,
    Lane 4: rat brain tissue lysates,
    Lane 5: rat liver tissue lysates,
    Lane 6: mouse brain tissue lysates,
    Lane 7: mouse liver tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alpha Tubulin/TUBA1B antigen affinity purified monoclonal antibody (BM3885) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha Tubulin/TUBA1B at approximately 55 kDa. The expected band size for Alpha Tubulin/TUBA1B is at 50 kDa.

    all(2)
  • Immunohistochemical analysis of paraffin-embedded human bladder, using alpha Tubulin Antibody.

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产品名称
Anti-Alpha Tubulin/TUBA1B Antibody (Clone#CG-20)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B; K-ALPHA-1
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
TUBA1B
克隆号
CG-20
抗体来源
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human alpha Tubulin
计算分子量
50 kDa
实际分子量
55 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Alpha-tubulin(b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.
Uniprot ID
文献引用格式
Alpha Tubulin/TUBA1B Antibody (Clone#CG-20) (Boster Biological Technology, Wuhan, China. Catalog#BM3885)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Tubulin alpha-1B chain
推荐稀释比
Western blot (WB):1:1000-5000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
ImmunoPrecipitation (IP):1:20
Flow Cytometry (FCM):1:20

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    Anti-Alpha Tubulin/TUBA1B Antibody (Clone#CG-20)

    筛选器: All WB IHC

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