Anti-a-SMA/ACTA2 Antibody (Clone#EO-1)

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF FCM

BM3902

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-a-SMA/ACTA2 antibody (BM3902). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human SH-SY5Y whole cell lysates,
Lane 7: human U251 whole cell lysates,
Lane 8: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM3902) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.
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Figure 2. Western blot analysis of anti-a-SMA/ACTA2 antibody (BM3902). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM3902) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody.
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Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody .
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-a-SMA/ACTA2 Antibody (Clone#EO-1)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

a SMA; AAT6; ACTA2; ACTA2 Smooth muscle Actin; Actin, aortic smooth muscle, Actin; aortic smooth muscle Actin; ACTSA; ACTVS; Alpha actin 2; Alpha SMA; a-SMA; sma; smooth muscle actin; α SMA; α-sma

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

ACTA2

克隆号

EO-1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human alpha smooth muscle Actin

计算分子量

42 kDa

实际分子量

42 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Uniprot ID

P62736

文献引用格式

a-SMA/ACTA2 Antibody (Clone#EO-1) (Boster Biological Technology, Wuhan, China. Catalog#BM3902)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

实验方案>

实验步骤

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