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Anti-S100 Beta/S100B Antibody (Clone#BCB-19)

Rabbit monoclonal antibody

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筛选器: All WB IHC ICC/IF IP

BM4087

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-S100 Beta/S100B antibody (BM4087). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat brain tissue lysates,
    Lane 2: rat C6 whole cell lysates,
    Lane 3: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100 Beta/S100B antigen affinity purified monoclonal antibody (BM4087) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for S100 Beta/S100B at approximately 11 kDa. The expected band size for S100 Beta/S100B is at 11 kDa.

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  • Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody.

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  • Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody.

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  • Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody.

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  • Immunohistochemical analysis of paraffin-embedded Mouse pancreas, using the Antibody.

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  • Immunohistochemical analysis of paraffin-embedded Mouse hippocampus , using the Antibody.

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  • Immunohistochemical analysis of paraffin-embedded rat brain, using S100B Antibody.

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  • Immunofluorescent analysis using the Antibody.

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  • Immunofluorescent analysis of A375 cells, using S100B Antibody .

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  • Early wheel-running promotes functional recovery by improving mitochondria metabolism in olfactory ensheathing cells after ischemic stroke in rats. -Behavioural Brain Research

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产品名称
Anti-S100 Beta/S100B Antibody (Clone#BCB-19)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
NEF; Protein S100 B; S 100 protein beta chain; S 100 protein subunit beta; S100; S100 Beta; S100 calcium binding protein B; S100B; S100beta
产品类型
Monoclonal
检验物种
human, mouse, rat, goat
应用范围
WB, IHC, ICC/IF, IP
基因名称
S100B
克隆号
BCB-19
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human S100B
计算分子量
11 kDa
实际分子量
11 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery. S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al.(1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.
Uniprot ID
P04271  
文献引用格式
S100 Beta/S100B Antibody (Clone#BCB-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4087)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Protein S100-B
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
ImmunoPrecipitation (IP):1:20

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    Anti-S100 Beta/S100B Antibody (Clone#BCB-19)

    筛选器: All WB IHC ICC/IF IP

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