Anti-S100 Beta/S100B Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PA1303

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of S100 Beta/S100B using anti-S100 Beta/S100B antibody (PA1303). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: Rat Brain tissue lysates,
Lane 3: MCF-7 whole cell lysates,
Lane 4: HELA whole cell lysates,
Lane 5: SMMC whole cell lysates,
Lane 6: JURKAT whole cell lysates,
Lane 7: COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100 Beta/S100B antigen affinity purified polyclonal antibody (PA1303) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for S100 Beta/S100B at approximately 11 kDa. The expected band size for S100 Beta/S100B is at 11 kDa.
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产品简介>

产品名称

Anti-S100 Beta/S100B Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NEF; Protein S100 B; S 100 protein beta chain; S 100 protein subunit beta; S100; S100 Beta; S100 calcium binding protein B; S100B; S100beta

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

S100B

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human S100 beta, identical to the related rat and mouse sequences.

计算分子量

11 kDa

实际分子量

11 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery. S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al.(1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.

Uniprot ID

P04271

RRID

AB_3082611

文献引用格式

S100 Beta/S100B Antibody (Boster Biological Technology, Wuhan, China. Catalog#PA1303)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

S100 calcium binding protein B

蛋白名全称

Protein S100-B

实验方案>

实验步骤

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