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Anti-Desmin/DES Antibody (Clone#BDF-4)

Rabbit monoclonal antibody

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筛选器: All WB IHC ICC/IF

BM4101

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-Desmin/DES antibody (BM4101). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat heart tissue lysates,
    Lane 2: rat skeletal muscle tissue lysates,
    Lane 3: mouse heart tissue lysates,
    Lane 4: mouse skeletal muscle tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Desmin/DES antigen affinity purified monoclonal antibody (BM4101) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Desmin/DES at approximately 54 kDa. The expected band size for Desmin/DES is at 54 kDa.

    all(3)
  • Immunohistochemical analysis of paraffin-embedded human prostate, using Desmin Antibody.

    all(3)

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产品名称
Anti-Desmin/DES Antibody (Clone#BDF-4)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CMD1I; CSM1; CSM2; Cytoskeleton Marker; DES; desmin
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF
基因名称
DES
克隆号
BDF-4
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human Desmin
计算分子量
54 kDa
实际分子量
54 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
DES, also called desmin, is a protein that in humans is encoded by the DES gene, and this gene is mapped to 2q35. DES is the muscle-specific member of the intermediate filament (IF) protein family. It is one of the earliest myogenic markers, both in heart and somites, and is expressed in satellite stem cells and replicating myoblasts. DES is very important in muscle cell architecture and structure since it connects many components of the cytoplasm. It may be also play an important role in mitochondria function. What’s more, DES provides attachments between the terminal Z disc and membrane-associated proteins to form a force-transmitting system that parallels the thin filaments at myotendinous junctions.
Uniprot ID
P17661  
文献引用格式
Desmin/DES Antibody (Clone#BDF-4) (Boster Biological Technology, Wuhan, China. Catalog#BM4101)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Desmin
推荐稀释比
Western blot (WB):1:1000-5000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200

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    Anti-Desmin/DES Antibody (Clone#BDF-4)

    筛选器: All WB IHC ICC/IF

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