Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
Western blot analysis of anti-Integrin Beta 3/ITGB3 antibody (BM4120). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Integrin Beta 3/ITGB3 antigen affinity purified monoclonal antibody (BM4120) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Beta 3/ITGB3 at approximately 100 kDa. The expected band size for Integrin Beta 3/ITGB3 is at 87 kDa.
Immunohistochemical analysis of paraffin-embedded human testis, using Integrin beta 3 Antibody .
Western blot analysis of anti-Integrin Beta 3/ITGB3 antibody (BM4120). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Integrin Beta 3/ITGB3 antigen affinity purified monoclonal antibody (BM4120) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Beta 3/ITGB3 at approximately 100 kDa. The expected band size for Integrin Beta 3/ITGB3 is at 87 kDa.
Immunohistochemical analysis of paraffin-embedded human testis, using Integrin beta 3 Antibody .