Anti-Osteopontin/SPP1 Antibody (Clone#CEO-19)

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF FCM

BM4208

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of Osteopontin/SPP1 using anti-Osteopontin/SPP1 antibody (BM4208). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse small intestine tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Osteopontin/SPP1 antigen affinity purified monoclonal antibody (BM4208) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Osteopontin/SPP1 at approximately 35 kDa. The expected band size for Osteopontin/SPP1 is at 35 kDa.
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Figure 2. IHC analysis of SPP1 using anti-SPP1 antibody (BM4208) .
SPP1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-SPP1 Antibody (BM4208) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Osteopontin/SPP1 Antibody (Clone#CEO-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BNSP; Bone sialoprotein 1; BSPI; ETA 1; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1; SPP 1; SPP1; Urinary stone protein; Uropontin

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC, ICC/IF, FCM

基因名称

SPP1

克隆号

CEO-19

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Osteopontin

计算分子量

35 kDa

实际分子量

35 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. And the encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. Also, this protein is a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

P10451

文献引用格式

Osteopontin/SPP1 Antibody (Clone#CEO-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4208)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Osteopontin

实验方案>

实验步骤

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