Anti-Osteopontin/SPP1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

PB0589

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Osteopontin/SPP1 using anti-Osteopontin/SPP1 antibody (PB0589). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: human SHG-44 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Osteopontin/SPP1 antigen affinity purified polyclonal antibody (PB0589) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Osteopontin/SPP1 at approximately 66 kDa(OPNprecursor),40 kDa(OPNcleaved). The expected band size for Osteopontin/SPP1 is at 35 kDa.
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产品名称

Anti-Osteopontin/SPP1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BNSP; Bone sialoprotein 1; BSPI; ETA 1; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1; SPP 1; SPP1; Urinary stone protein; Uropontin

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

SPP1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Osteopontin different from the related mouse sequence by eight amino acids, and from the related rat sequence by seven amino acids.

计算分子量

35 kDa

实际分子量

66 kDa(OPNprecursor),40 kDa(OPNcleaved)

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. And the encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. Also, this protein is a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

P10451

文献引用格式

Osteopontin/SPP1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0589)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Osteopontin

实验方案>

实验步骤

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