Figure 1. Western blot analysis of anti-SOX9 antibody (BM4268). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOX9 antigen affinity purified monoclonal antibody (BM4268) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOX9 at approximately 70 kDa. The expected band size for SOX9 is at 56 kDa.
Figure 2. IHC analysis of SOX9 using anti-SOX9 antibody (BM4268) .
SOX9 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of SOX9 using anti-SOX9 antibody (BM4268) .
SOX9 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of SOX9 using anti-SOX9 antibody (BM4268) .
SOX9 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of SOX9 using anti-SOX9 antibody (BM4268).
SOX9 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
Figure 1. Western blot analysis of anti-SOX9 antibody (BM4268). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOX9 antigen affinity purified monoclonal antibody (BM4268) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOX9 at approximately 70 kDa. The expected band size for SOX9 is at 56 kDa.
Figure 2. IHC analysis of SOX9 using anti-SOX9 antibody (BM4268) .
SOX9 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of SOX9 using anti-SOX9 antibody (BM4268) .
SOX9 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of SOX9 using anti-SOX9 antibody (BM4268) .
SOX9 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of SOX9 using anti-SOX9 antibody (BM4268).
SOX9 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
Figure 1. Western blot analysis of anti-SOX9 antibody (BM4268). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOX9 antigen affinity purified monoclonal antibody (BM4268) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOX9 at approximately 70 kDa. The expected band size for SOX9 is at 56 kDa.
Figure 2. IHC analysis of SOX9 using anti-SOX9 antibody (BM4268) .
SOX9 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of SOX9 using anti-SOX9 antibody (BM4268) .
SOX9 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of SOX9 using anti-SOX9 antibody (BM4268) .
SOX9 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of SOX9 using anti-SOX9 antibody (BM4268).
SOX9 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SOX9 Antibody (BM4268) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
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