Western blot (WB): | 1:2000 |
Immunohistochemistry (IHC): | 1:150 |
Figure 1. Western blot analysis of HT29 cell lysate (35ug) by using anti-SOX9 monoclonal antibody. Dilution: 1:500
Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX9(Cat# M00177-1).
Figure 3. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00177-1)
Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00177-1)
Figure 5. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00177-1)
Figure 6. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00177-1)
Figure 1. Western blot analysis of HT29 cell lysate (35ug) by using anti-SOX9 monoclonal antibody. Dilution: 1:500
Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX9(Cat# M00177-1).
Figure 3. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00177-1)
Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00177-1)
Figure 5. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00177-1)
Figure 6. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00177-1)