Anti-CDK1 (Phospho-T14) Antibody (Clone#DFH-3)

Rabbit Monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC IP

BM4328

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

加入购物车立即购买

Figure 1. Western blot analysis of anti-CDK1 (Phospho-T14) antibody (BM4328). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDK1 (Phospho-T14) antigen affinity purified monoclonal antibody (BM4328) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK1 (Phospho-T14) at approximately 34 kDa. The expected band size for CDK1 (Phospho-T14) is at 34 kDa.
all(3)
Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody.
all(3)
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CDK1 (Phospho-T14) Antibody (Clone#DFH-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CDC2; CDC28A; CDK1; Cell division protein kinase 1; Cyclin dependent kinase 1; p34 protein kinase

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

CDK1

克隆号

DFH-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-Cdk1/2 (T14)

计算分子量

34 kDa

实际分子量

34 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The cyclin-dependent protein kinases(CDKs) regulate major cell cycle transitions in eukaryotic cells. CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR(EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIRE motif found in prototypic CDC2 kinases. CDC2L1 is referred as PITSLRE B, based on the amino acid sequence of the region corresponding to the conserved CDC2 PSTAIRE box.

Uniprot ID

P06493/P24941

文献引用格式

CDK1 (Phospho-T14) Antibody (Clone#DFH-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4328)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Cyclin-dependent kinase 1

实验方案>

实验步骤

引用文献(0)>

清除