Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti- PSTAIR antibody (MA1087). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: 293T whole cell lysates,
Lane 3: Caco-2 whole cell lysates,
Lane 4: THP-1 whole cell lysates,
Lane 5: C6 whole cell lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: RAW264.7 whole cell lysates,
Lane 8: NIH/3T3 whole cell lysates.
Use mouse anti- PSTAIR 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PSTAIR at approximately 34KD. The expected band size for PSTAIR is at 34KD.
Figure 1. Western blot analysis of anti- PSTAIR antibody (MA1087). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: 293T whole cell lysates,
Lane 3: Caco-2 whole cell lysates,
Lane 4: THP-1 whole cell lysates,
Lane 5: C6 whole cell lysates,
Lane 6: PC-12 whole cell lysates,
Lane 7: RAW264.7 whole cell lysates,
Lane 8: NIH/3T3 whole cell lysates.
Use mouse anti- PSTAIR 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PSTAIR at approximately 34KD. The expected band size for PSTAIR is at 34KD.