产品名称
Anti-NSE/ENO2 Antibody (Clone#FBI-5)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
enolase 2 (gamma; neuronal); Gamma enolase; Neural enolase; Neuron specific enolase; NSE
应用范围
WB, IHC, ICC/IF, FCM
免疫原
A synthesized peptide derived from human NSE
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
NSE (neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.
文献引用格式
NSE/ENO2 Antibody (Clone#FBI-5) (Boster Biological Technology, Wuhan, China. Catalog#BM4495)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
Western blot (WB): | 1:1000-5000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
Flow Cytometry (FCM): | 1:20 |