Anti-MEK1/MAP2K1 (Phospho-S298) Antibody (Clone#HBO-13)

Rabbit Monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4690

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-MEK1/MAP2K1 (Phospho-S298) antibody (BM4690). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MEK1/MAP2K1 (Phospho-S298) antigen affinity purified monoclonal antibody (BM4690) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MEK1/MAP2K1 (Phospho-S298) at approximately 45 kDa. The expected band size for MEK1/MAP2K1 (Phospho-S298) is at 43 kDa.
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产品简介>

产品名称

Anti-MEK1/MAP2K1 (Phospho-S298) Antibody (Clone#HBO-13)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MKK1; PRKMK1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

MAP2K1

克隆号

HBO-13

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human MEK1

计算分子量

43 kDa

实际分子量

45 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Dual specificity mitogen-activated protein kinase kinase 1 is an enzyme that in humans is encoded by the MAP2K1 gene. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein(MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon activation by a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Rampoldi et al.(1997) localized the MAP2K1 gene to 15q22.1-q22.33.

Uniprot ID

Q02750

文献引用格式

MEK1/MAP2K1 (Phospho-S298) Antibody (Clone#HBO-13) (Boster Biological Technology, Wuhan, China. Catalog#BM4690)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Dual specificity mitogen-activated protein kinase kinase 1

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实验步骤

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