Anti-MEK1/MAP2K1 Antibody (Clone#OTI2D4)

Mouse monoclonal antibody

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M00292-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00292-4)
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAP2K1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00292-4)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00292-4)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00292-4)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00292-4)
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Anti-MAP2K1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K1 .
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1.
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产品简介>

产品名称

Anti-MEK1/MAP2K1 Antibody (Clone#OTI2D4)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MKK1; PRKMK1

产品类型

Monoclonal

检验物种

human, mouse, rat, dog, monkey

应用范围

WB, IHC, ICC/IF

基因名称

MAP2K1

克隆号

OTI2D4

宿主

Mouse

抗体亚型

IgG2a

免疫原

Full length human recombinant protein of human MAP2K1(NP_002746) produced in HEK293T cell.

计算分子量

43 kDa

实际分子量

43.3 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq]

Uniprot ID

Q02750

文献引用格式

MEK1/MAP2K1 Antibody (Clone#OTI2D4) (Boster Biological Technology, Wuhan, China. Catalog#M00292-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

mitogen-activated protein kinase kinase

蛋白名全称

Dual specificity mitogen-activated protein kinase kinase 1

实验方案>

实验步骤

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