Anti-PRDX1 Antibody (Clone#IIG-16)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM4871

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-PRDX1 antibody (BM4871). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRDX1 antigen affinity purified monoclonal antibody (BM4871) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PRDX1 at approximately 24 kDa. The expected band size for PRDX1 is at 22 kDa.
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产品简介>

产品名称

Anti-PRDX1 Antibody (Clone#IIG-16)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

MSP23; NKEF A; NKEFA; PAG; PAGA; PAGB; peroxiredoxin 1; PRDX1; PRX1; PRXI; TDPX2; Thioredoxin peroxidase 2

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC, ICC/IF, FCM

基因名称

PRDX1

克隆号

IIG-16

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human PRDX1

计算分子量

22 kDa

实际分子量

22 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PRDX1(Peroxiredoxin 1), also called PRX1, PAGA or NKEFA, is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The PRDX1 gene is mapped on 1p34.1. Prdx1 was expressed in differentiating motor neuron cells in developing embryonic chicken and mouse spinal cords. mmunoprecipitation analysis showed that GDE2 interacted directly with PRDX1 in embryonic chicken spinal cord extracts and in transfected HEK293T cells. This protein may have a proliferative effect and play a role in cancer development or progression. In differentiating spinal cord, Prdx1 was required to activate Gde2 by reducing an intramolecular cystine bridge between the Gde2 N- and C-terminal domains. An intramolecular disulfide bond between the GDE2 N- and C-terminal domains inhibits GDE2 function, and that reduction of this cystine by PRDX1 activates GDE2 for the induction of motor neuron differentiation.

Uniprot ID

Q06830

文献引用格式

PRDX1 Antibody (Clone#IIG-16) (Boster Biological Technology, Wuhan, China. Catalog#BM4871)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Peroxiredoxin-1

实验方案>

实验步骤

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