Anti-PRDX1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

PB9348

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-PRDX1 antibody (PB9348). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MDA-MB-453 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human HEK293 whole cell lysates,
Lane 7: human placenta tissue lysates,
Lane 8: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRDX1 antigen affinity purified polyclonal antibody (PB9348) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PRDX1 at approximately 24 kDa. The expected band size for PRDX1 is at 22 kDa.
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Western blot analysis of anti-PRDX1 antibody (PB9348). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRDX1 antigen affinity purified polyclonal antibody (PB9348) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PRDX1 at approximately 24 kDa. The expected band size for PRDX1 is at 22 kDa.
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IHC analysis of PRDX1 using anti-PRDX1 antibody (PB9348).
PRDX1 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of PRDX1 using anti-PRDX1 antibody (PB9348).
PRDX1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of PRDX1 using anti-PRDX1 antibody (PB9348).
PRDX1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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ICC analysis of PRDX1 using anti-PRDX1 antibody (PB9348).
PRDX1 was detected in an immunocytochemical section of SMMC-7721 cells. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of PRDX1 using anti-PRDX1 antibody (PB9348).
PRDX1 was detected in an immunocytochemical section of Hela cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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IF analysis of PRDX1 using anti-PRDX1 antibody (PB9348).
PRDX1 was detected in an immunocytochemical section of U2OS cells. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of HepG2 cells using anti-PRDX1 antibody (PB9348).
Overlay histogram showing HepG2 cells stained with PB9348 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDX1 Antibody (PB9348) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-PRDX1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

MSP23; NKEF A; NKEFA; PAG; PAGA; PAGB; peroxiredoxin 1; PRDX1; PRX1; PRXI; TDPX2; Thioredoxin peroxidase 2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

PRDX1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Peroxiredoxin 1, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

计算分子量

22 kDa

实际分子量

22 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PRDX1(Peroxiredoxin 1), also called PRX1, PAGA or NKEFA, is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The PRDX1 gene is mapped on 1p34.1. Prdx1 was expressed in differentiating motor neuron cells in developing embryonic chicken and mouse spinal cords. mmunoprecipitation analysis showed that GDE2 interacted directly with PRDX1 in embryonic chicken spinal cord extracts and in transfected HEK293T cells. This protein may have a proliferative effect and play a role in cancer development or progression. In differentiating spinal cord, Prdx1 was required to activate Gde2 by reducing an intramolecular cystine bridge between the Gde2 N- and C-terminal domains. An intramolecular disulfide bond between the GDE2 N- and C-terminal domains inhibits GDE2 function, and that reduction of this cystine by PRDX1 activates GDE2 for the induction of motor neuron differentiation.

Uniprot ID

Q06830

文献引用格式

PRDX1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9348)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

peroxiredoxin 1

蛋白名全称

Peroxiredoxin-1

实验方案>

实验步骤

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