Western blot (WB): | 1:500-2000 |
Western blot analysis of anti-COL10A1 antibody (BM4893). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COL10A1 antigen affinity purified monoclonal antibody (BM4893) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COL10A1 at approximately 66 kDa. The expected band size for COL10A1 is at 66 kDa.
Western blot analysis of anti-COL10A1 antibody (BM4893). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COL10A1 antigen affinity purified monoclonal antibody (BM4893) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COL10A1 at approximately 66 kDa. The expected band size for COL10A1 is at 66 kDa.