Anti-PGC1 Alpha/PPARGC1A Antibody (Clone#AOBC-16)

Rabbit monoclonal antibody

说明书

筛选器： All WB ICC/IF

BM4898

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of anti-PGC1 Alpha/PPARGC1A antibody (BM4898). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PGC1 Alpha/PPARGC1A antigen affinity purified monoclonal antibody (BM4898) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PGC1 Alpha/PPARGC1A at approximately 113 kDa. The expected band size for PGC1 Alpha/PPARGC1A is at 91 kDa.
all(3)
Figure 2. Western blot analysis of anti-PGC1 Alpha/PPARGC1A antibody (BM4898). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PGC1 Alpha/PPARGC1A antigen affinity purified monoclonal antibody (BM4898) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PGC1 Alpha/PPARGC1A at approximately 113 kDa. The expected band size for PGC1 Alpha/PPARGC1A is at 91 kDa.
all(3)
Immunofluorescent analysis using the Antibody.
all(3)

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产品简介>

产品名称

Anti-PGC1 Alpha/PPARGC1A Antibody (Clone#AOBC-16)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

LEM6; Ligand effect modulator 6; PGC 1 alpha; PGC 1(alpha); PGC 1v; PGC1; PGC1A; PGC-1α; PPAR gamma coactivator 1 alpha; PPARGC 1 alpha; PPARGC1; PPARGC1A; PPARGC-1-alpha; PGC-1-alpha

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

PPARGC1A

克隆号

AOBC-16

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human PGC1 alpha

计算分子量

91 kDa

实际分子量

113 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.

Uniprot ID

Q9UBK2

文献引用格式

PGC1 Alpha/PPARGC1A Antibody (Clone#AOBC-16) (Boster Biological Technology, Wuhan, China. Catalog#BM4898)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha

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实验步骤

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