Anti-MFN2 Antibody (Clone#AOCA-13)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4906

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-MFN2 antibody (BM4906). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MFN2 antigen affinity purified monoclonal antibody (BM4906) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MFN2 at approximately 86 kDa. The expected band size for MFN2 is at 86 kDa.
all(3)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Mitofusin 2 Antibody.
all(3)
Immunofluorescent analysis of Hela cells, using Mitofusin 2 Antibody.
all(3)

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产品名称

Anti-MFN2 Antibody (Clone#AOCA-13)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CMT2A; CMT2A2; CPRP1; HSG; KIAA0214; MARF; MFN2; Mitofusin 2; Transmembrane GTPase MFN2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

MFN2

克隆号

AOCA-13

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Mitofusin 2

计算分子量

86 kDa

实际分子量

86 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Mitofusin 2(MFN2) is a mitochondrial transmembrane GTPase regulating mitochondrial fusion and that the nucleotide-dependent activation of MFN2 concomitantly protects the organelle from permeability transition. It is mapped to chromosome 1 and encodes a 757-amino acid protein that contains an ATP/GTP-binding site motif. It is expressed in many tissues and cell lines such as brain and KG-1 with the highest expression in heart and skeletal muscle. This protein contains an N-terminal GTPase domain and a transmembrane domain near the C terminus. It shares 60% identity with MFN1. When stably expressed in COS-7 cells, MFN2 colocalizes with mitochondrial markers. Axonal CMT type 2A and autosomal dominant HMSN VI are caused by MFN2 and mutations in MFN2, which emphasizes its important role of mitochondrial function for both optic atrophies and peripheral neuropathies.

Uniprot ID

O95140

文献引用格式

MFN2 Antibody (Clone#AOCA-13) (Boster Biological Technology, Wuhan, China. Catalog#BM4906)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Mitofusin-2

实验方案>

实验步骤

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