Anti-MFN2 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB

PB0264

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of MFN2 using anti-MFN2 antibody (PB0264).
Lane 1: recombinant Human Mitofusin-2 Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MFN2 antigen affinity purified polyclonal antibody (PB0264) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MFN2 at approximately 45 kDa.
all(3)
Western blot analysis of MFN2 using anti-MFN2 antibody (PB0264). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MFN2 antigen affinity purified polyclonal antibody (PB0264) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MFN2 at approximately 86 kDa. The expected band size for MFN2 is at 86 kDa.
all(3)
Western blot analysis of MFN2 using anti-MFN2 antibody (PB0264). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse small intestine tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MFN2 antigen affinity purified polyclonal antibody (PB0264) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MFN2 at approximately 86 kDa. The expected band size for MFN2 is at 86 kDa.
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-MFN2 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CMT2A; CMT2A2; CPRP1; HSG; KIAA0214; MARF; MFN2; Mitofusin 2; Transmembrane GTPase MFN2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

MFN2

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Mitofusin 2 recombinant protein (Position: V601-R757). Human Mitofusin 2 shares 96% and 95% amino acid (aa) sequence identity with mouse and rat Mitofusin 2, respectively.

计算分子量

86 kDa

实际分子量

86 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Mitofusin-2 is a protein that in humans is encoded by the MFN2 gene. It is mapped to chromosome 1 and encodes a 757-amino acid protein that contains an ATP/GTP-binding site motif. This gene is expressed in many tissues and cell lines such as brain and KG-1 with the highest expression in heart and skeletal muscle. It has been found that MFN2 triggers mitochondrial energization, at least in part, by regulating OXPHOS expression through signals that are independent of its role as a mitochondrial fusion protein. And it contributes to the maintenance and operation of the mitochondrial network. Axonal CMT type 2A and autosomal dominant HMSN VI are caused by MFN2 and mutations in MFN2, which emphasizes its important role of mitochondrial function for both optic atrophies and peripheral neuropathies.

Uniprot ID

O95140

文献引用格式

MFN2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0264)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

mitofusin 2

蛋白名全称

Mitofusin-2

实验方案>

实验步骤

引用文献(0)>

清除