Anti-ATF4 Antibody (Clone#HCI-1)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF IP FCM

BM5179

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-ATF4 antibody (BM5179). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF4 antigen affinity purified monoclonal antibody (BM5179) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF4 at approximately 55 kDa. The expected band size for ATF4 is at 39 kDa.
all(8)
Immunohistochemical analysis of paraffin-embedded mouse kidney, using ATF4 Antibody.
all(8)
Immunofluorescent analysis using the Antibody.
all(8)
Immunofluorescent analysis using the Antibody.
all(8)
Immunofluorescent analysis using the Antibody.
all(8)
Immunofluorescent analysis of 293 cells, using ATF4 Antibody.
all(8)
all(8)
all(8)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ATF4 Antibody (Clone#HCI-1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ATF4; CREB 2; CREB2; DNA binding protein TAXREB67; TAXREB67; TXREB

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

ATF4

克隆号

HCI-1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ATF4

计算分子量

39 kDa

实际分子量

45-50 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ATF4, Activating Transcription Factor 4, is also known as CREB2. ATF4 belongs to the large ATF/CREB family of transcription factors which bind DNA via their basic region and dimerize via their leucine zipper domain to form a variety of homo- and heterodimers to regulate gene transcription. It is identified that members of this family share significant sequence similarity within a leucine zipper DNA-binding motif and an adjacent basic region. The ATF4 gene is mapped to chromosome 22. Unlike CREB, which activates transcription from CRE-containing promoters, CREB2 functions as a specific repressor of CRE-dependent transcription. The transcriptional repressor activity resides within the C-terminal leucine zipper and basic domain region of the CREB2 protein.

Uniprot ID

P18848

文献引用格式

ATF4 Antibody (Clone#HCI-1) (Boster Biological Technology, Wuhan, China. Catalog#BM5179)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Cyclic AMP-dependent transcription factor ATF-4

实验方案>

实验步骤

引用文献(0)>

清除