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Anti-EIF4A1 Antibody (Clone#18E95)

Rabbit monoclonal antibody

说明书

筛选器: All WB ICC/IF FCM

BM5594

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Figure 1. Western blot analysis of anti-EIF4A1 antibody (BM5594). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human MCF-7 whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: human 293T whole cell lysates,
    Lane 4: rat PC-12 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EIF4A1 antigen affinity purified monoclonal antibody (BM5594) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EIF4A1 at approximately 46 kDa. The expected band size for EIF4A1 is at 46 kDa.

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产品名称
Anti-EIF4A1 Antibody (Clone#18E95)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Eukaryotic initiation factor 4A-I; eIF-4A-I; EIF4A-I; ATP-dependent RNA helicase eIF4A-1; EIF4A1; DDX2A; EIF4A
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, ICC/IF, FCM
基因名称
EIF4A1
克隆号
18E95
抗体来源
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human eIF4A1
计算分子量
46 kDa
实际分子量
46 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Eukaryotic initiation factor 4A-I is a protein that in humans is encoded by the EIF4A1 gene. It is mapped to 17p13.1. EIF4A1 has been shown to interact with EIF4E and eukaryotic translation initiation factor 4 gamma.
Uniprot ID
P60842  
文献引用格式
EIF4A1 Antibody (Clone#18E95) (Boster Biological Technology, Wuhan, China. Catalog#BM5594)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
Flow Cytometry (FCM):1:50

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    Anti-EIF4A1 Antibody (Clone#18E95)

    筛选器: All WB ICC/IF FCM

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