Bovine BMP2 ELISA Kit

96T/2480

BMP-2; BMP 2; BMP 2A; BMP2; BMP2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A

Expression system for standard: CHO; Immunogen sequence: Q283-R396

There is no detectable cross-reactivity with other relevant proteins.

<2pg/ml

31.2pg/ml-2000pg/ml

BMP2

bone morphogenetic protein 2

C8C060

Bovine BMP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0311-BV)

Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant Bovine BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

15-20分钟

Catalog number	Description	Quantity
EK0311-BV-CAP	Anti-Bovine BMP2 Pre-coated 96-well strip microplate	1
EK0311-BV-ST	Bovine BMP2 Standard	10ng/tube×2
EK0311-BV-DA	Bovine BMP2 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	285	742	1197	306	834	1358
Standard deviation	9.12	23.17	37.12	18.97	58.38	97.78
CV (%)	3.2	3.5	3.1	6.2	7	7.2

Concentration(pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.005	0.034	0.059	0.119	0.277	0.68	1.642	2.417

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

bovine

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum