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Rat BMP2 ELISA Kit

说明书

EK0312

  • 96T ¥2480
  • 货期: 现货

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产品名称
Rat BMP2 ELISA Kit
规格/价格
96T/2480
指标别名
BMP-2; BMP 2; BMP 2A; BMP2; BMP2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A
免疫原
Expression system for standard: CHO; Immunogen sequence: Q283-R396
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2pg/ml-2000pg/ml
基因名称
BMP2
基因名全称
bone morphogenetic protein 2
Uniprot ID
P49001  
文献引用格式
Rat BMP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0312)
背景资料
Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
试剂盒内容
Catalog numberDescriptionQuantity
EK0312-CAP96-well plate precoated with anti- rat BMP-2 antibody1
EK0312-STLyophilized recombinant rat BMP-2 standard10ng/tube×2
EK0312-DABiotinylated anti- rat BMP-2 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)96245806105234777
Standard deviation6.7211.2749.169.2414.9752.83
CV(%)7%4.6%6.1%8.8%6.4%6.8%
典型数据示例
Concentration(pg/ml)031.262.512525050010002000
O.D.0.0170.0690.1160.2150.4540.9922.0082.506
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
rat
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants and Serum

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