指标别名
BMP-2; BMP 2; BMP 2A; BMP2; BMP2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A
免疫原
Expression system for standard: CHO; Immunogen sequence: Q283-R396
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
bone morphogenetic protein 2
文献引用格式
Rat BMP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0312)
背景资料
Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
试剂盒内容
Catalog number | Description | Quantity |
EK0312-CAP | 96-well plate precoated with anti- rat BMP-2 antibody | 1 |
EK0312-ST | Lyophilized recombinant rat BMP-2 standard | 10ng/tube×2 |
EK0312-DA | Biotinylated anti- rat BMP-2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 96 | 245 | 806 | 105 | 234 | 777 |
Standard deviation | 6.72 | 11.27 | 49.16 | 9.24 | 14.97 | 52.83 |
CV(%) | 7% | 4.6% | 6.1% | 8.8% | 6.4% | 6.8% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.017 | 0.069 | 0.116 | 0.215 | 0.454 | 0.992 | 2.008 | 2.506 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum