Human BMP4 ELISA Kit

96T/2480

BMP 2B; BMP 4; BMP2B; BMP2B1; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; NeutraKine® BMP-4; ZYME

NSO, S293-R408

There is no detectable cross-reactivity with other relevant proteins.

62.5 pg/ml - 4000 pg/ml

BMP4

bone morphogenetic protein 4

P12644  

Human BMP4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0314)

Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog(released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal(by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.

15-20分钟

Catalog number	Description	Quantity
EK0314-CAP	96-well plate precoated with anti- human BMP-4 antibody	1
EK0314-ST	Lyophilized recombinant human BMP-4 standard	10ng/tube×2
EK0314-DA	Biotinylated anti- human BMP-4 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	196	814	1756	199	823	1888
Standard deviation	12.15	48.02	124.67	12.33	49.38	169.92
CV(%)	6.2	5.9	7.1	6.2	6	9

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.037	0.083	0.143	0.285	0.523	1.037	1.678	2.412

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants

3.5h