Bovine BMP4 ELISA Kit

96T/2480

BMP 2B; BMP 4; BMP2B; BMP2B1; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; NeutraKine® BMP-4; ZYME

Expression system for standard: NS0; Immunogen sequence: S293-R408

There is no detectable cross-reactivity with other relevant proteins.

62.5pg/ml-4000pg/ml

BMP4

Bone morphogenetic protein 4

P12644  

Bovine BMP4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0314-BV)

Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In Bovine embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog(released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal(by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.

15-20分钟

Catalog number	Description	Quantity
EK0314-BV-CAP	Anti-Bovine BMP4 Pre-coated 96-well strip microplate	1
EK0314-BV-ST	Bovine BMP4 Standard	10ng/tube×2
EK0314-BV-DA	Bovine BMP4 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	376	987	2136	453	1209	2351
Standard deviation	45.409	45.40	117.48	23.10	68.91	141.06
CV (%)	4.2	4.6	5.5	6.0	5.7	6.0

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.037	0.083	0.143	0.285	0.523	1.037	1.678	2.412

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

bovine

酶联免疫双抗夹心法

Cell Culture Supernatants