Human EGF ELISA Kit

96T/1880

EGF; HOMG4; Pro epidermal growth factor; URG; Urogastrone

E.coli, N971-R1023

There is no detectable cross-reactivity with other relevant proteins.

4.7 pg/ml - 300 pg/ml

EGF

epidermal growth factor

P01133  

Human EGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0325)

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

15-20分钟

Catalog number	Description	Quantity
EK0325-CAP	96-well plate precoated with anti- human EGF antibody	1
EK0325-ST	Lyophilized recombinant human EGF standard	1ng/tube×2
EK0325-DA	Biotinylated anti- human EGF antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	11	76	122	10	81	131
Standard deviation	0.71	4.25	9.15	0.82	5.42	12.31
CV(%)	6.5	5.6	7.5	8.2	6.7	9.4

Concentration(pg/ml)	0	4.7	9.4	18.8	37.5	75	150	300
O.D.	0.046	0.092	0.151	0.265	0.521	0.943	1.504	1.965

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine

3.5h