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Rat EGF ELISA Kit

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EK0954

  • 96T ¥2480
  • 货期: 现货

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产品名称
Rat EGF ELISA Kit
规格/价格
96T/2480
指标别名
EGF; HOMG4; Pro epidermal growth factor; URG; Urogastrone
免疫原
Expression system for standard: E.coli; Immunogen sequence: N974-R1026
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
7.8pg/ml-500pg/ml
基因名称
EGF
基因名全称
epidermal growth factor
Uniprot ID
P07522  
文献引用格式
Rat EGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0954)
背景资料
Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0954-CAP96-well plate precoated with anti- rat EGF antibody1
EK0954-STLyophilized recombinant rat EGF standard10ng/tube×2
EK0954-DABiotinylated anti- rat EGF antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)11902321096211
Standard deviation0.755.3116.240.836.9116.66
CV(%)6.9%5.9%7%8.3%7.2%7.9%
典型数据示例
Concentration(pg/ml)07.815.631.362.5125250500
O.D.0.0330.1290.2070.4060.7151.2102.0122.550
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
rat
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA)and Urine

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