指标别名
EGF; HOMG4; Pro epidermal growth factor; URG; Urogastrone
免疫原
Expression system for standard: E.coli; Immunogen sequence: N974-R1026
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
epidermal growth factor
文献引用格式
Rat EGF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0954)
背景资料
Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.
试剂盒内容
Catalog number | Description | Quantity |
EK0954-CAP | 96-well plate precoated with anti- rat EGF antibody | 1 |
EK0954-ST | Lyophilized recombinant rat EGF standard | 10ng/tube×2 |
EK0954-DA | Biotinylated anti- rat EGF antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 11 | 90 | 232 | 10 | 96 | 211 |
Standard deviation | 0.75 | 5.31 | 16.24 | 0.83 | 6.91 | 16.66 |
CV(%) | 6.9% | 5.9% | 7% | 8.3% | 7.2% | 7.9% |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.033 | 0.129 | 0.207 | 0.406 | 0.715 | 1.210 | 2.012 | 2.550 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA)and Urine