Human EGFR ELISA Kit

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EK0327

96T ￥2480
货期：现货

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Human EGFR ELISA Kit standard curve
all(2)
all(2)

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产品简介>

产品名称

Human EGFR ELISA Kit

规格/价格

96T/2480

指标别名

EGFR; ERBB; ERBB1; HER1; mENA; PIG61; Proto oncogene c ErbB 1

免疫原

NSO, M1-S645

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

156 pg/ml - 10000 pg/ml

基因名称

EGFR

基因名全称

Epidermal growth factor receptor

Uniprot ID

P00533

文献引用格式

Human EGFR ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0327)

背景资料

The epidermal growth factor receptor(EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family(EGF-family) of extracellular protein ligands.1 It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR(ErbB-1), HER2/c-neu(ErbB-2), Her 3(ErbB-3) and Her 4(ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha(TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression(known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0327-CAP	Anti-Human EGFR Pre-coated 96-well strip microplate	1
EK0327-ST	Human EGFR Standard	10ng/tube×2
EK0327-DA	Human EGFR Biotinylated antibody (100x)	100µl
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100µl
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(ng/ml)	1.37	4.68	6.21	1.49	4.92	7.14
Standard deviation	0.053	0.211	0.329	0.073	0.32	0.614
CV(%)	3.9	4.5	5.3	4.7	6.5	8.6

典型数据示例

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.040	0.168	0.287	0.378	0.689	1.282	1.566	2.308

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human EGFR ELISA Kit

EK0327

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

蛋白名全称

检测方法

样本类型

实验时长

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Human EGFR ELISA Kit

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