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Mouse EGFR ELISA Kit

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EK1433

  • 96T ¥2480
  • 货期: 现货

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产品名称
Mouse EGFR ELISA Kit
规格/价格
96T/2480
指标别名
EGFR; ERBB; ERBB1; HER1; mENA; PIG61; Proto oncogene c ErbB 1
免疫原
Expression system for standard: NS0; Immunogen sequence: L25-S647
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156pg/ml-10000pg/ml
基因名称
Egfr
基因名全称
Epidermal growth factor receptor
Uniprot ID
Q01279  
文献引用格式
Mouse EGFR ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1433)
背景资料
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.
试剂盒内容
Catalog numberDescriptionQuantity
EK1433-CAP96-well plate precoated with anti- mouse EGFR antibody1
EK1433-STLyophilized recombinant mouse EGFR standard10ng/tube×2
EK1433-DABiotinylated anti- mouse EGFR antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3141163535833911465763
Standard deviation16.9560.47241.1121.0175.63305.43
CV(%)5.4%5.2%4.5%6.2%6.6%5.3%
典型数据示例
Concentration(pg/ml)015631262512502500500010000
O.D.0.0100.0790.1530.2760.4781.0171.4702.147
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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