产品名称
Mouse EGFR ELISA Kit
指标别名
EGFR; ERBB; ERBB1; HER1; mENA; PIG61; Proto oncogene c ErbB 1
免疫原
Expression system for standard: NS0; Immunogen sequence: L25-S647
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Epidermal growth factor receptor
文献引用格式
Mouse EGFR ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1433)
背景资料
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1433-CAP | 96-well plate precoated with anti- mouse EGFR antibody | 1 |
EK1433-ST | Lyophilized recombinant mouse EGFR standard | 10ng/tube×2 |
EK1433-DA | Biotinylated anti- mouse EGFR antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 314 | 1163 | 5358 | 339 | 1146 | 5763 |
Standard deviation | 16.95 | 60.47 | 241.11 | 21.01 | 75.63 | 305.43 |
CV(%) | 5.4% | 5.2% | 4.5% | 6.2% | 6.6% | 5.3% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.010 | 0.079 | 0.153 | 0.276 | 0.478 | 1.017 | 1.470 | 2.147 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)