Human EPO ELISA Kit

96T/2480

EP; EPO; Epoetin; erythropoietin; MVCD2

CHO, A28-R193

There is no detectable cross-reactivity with other relevant proteins.

3.12 mIU/ml - 200 mIU/ml

EPO

erythropoietin

P01588  

Human EPO ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0332)

Erythropoietin(EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. It is mapped to 7q22.1. This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.

15-20分钟

Catalog number	Description	Quantity
EK0332-CAP	96-well plate precoated with anti- human EPO antibody	1
EK0332-ST	Lyophilized recombinant human EPO standard	200mIU/tube×2
EK0332-DA	Biotinylated anti- human EPO antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	4	23	68	5	24	76
Standard deviation	0.17	0.99	2.79	0.33	1.63	5.47
CV(%)	4.2	4.3	4.1	6.6	6.8	7.2

Concentration(mIU/ml)	0	3.12	6.25	12.5	25	50	100	200
O.D.	0.049	0.176	0.236	0.415	0.671	1.097	1.657	2.186

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

3.5h