指标别名
EP; EPO; Epoetin; erythropoietin; MVCD2
免疫原
Expression system for standard: NS0; Immunogen sequence: A27-R192
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse EPO ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0333)
背景资料
Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0333-CAP | 96-well plate precoated with anti- mouse EPO antibody | 1 |
EK0333-ST | Lyophilized recombinant mouse EPO standard | 10ng/tube×2 |
EK0333-DA | Biotinylated anti- mouse EPO antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 111 | 307 | 1298 | 106 | 278 | 1216 |
Standard deviation | 7.88 | 17.19 | 55.81 | 8.26 | 16.68 | 71.74 |
CV(%) | 7.1% | 5.6% | 4.3% | 7.8% | 6% | 5.9% |
典型数据示例
Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.020 | 0.065 | 0.101 | 0.173 | 0.338 | 0.730 | 1.524 | 2.312 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)