Human Fractalkine/CX3CL1 ELISA Kit

96T/2480

ABCD 3; C X3 C motif chemokine 1; C3Xkine; CX3CL1; CXC3; CXC3C; FKN; fractalkine; neurotactin; NTN; NTT; SCYD1; Small inducible cytokine D1

NSO, Q25-R339

There is detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC< 0.2%.

156 pg/ml - 10000 pg/ml

CX3CL1

chemokine (C-X3-C motif) ligand 1

P78423  

Human Fractalkine/CX3CL1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0356)

Chemokine(C-X3-C motif) ligand 1(CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). CX3CL1 is produced as a long protein(with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble(90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.

15-20分钟

Catalog number	Description	Quantity
EK0356-CAP	96-well plate precoated with anti- human Fractalkine antibody	1
EK0356-ST	Lyophilized recombinant human Fractalkine standard	10ng/tube×2
EK0356-DA	Biotinylated anti- human Fractalkine antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	296	1773	5345	269	1730	5847
Standard deviation	16.87	90.42	315.35	17.75	103.8	374.2
CV(%)	5.7	5.1	5.9	6.6	6	6.4

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.089	0.239	0.349	0.546	0.772	1.198	1.649	2.126

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk

3.5h