Mouse Fractalkine/CX3CL1 ELISA Kit

96T/2480

ABCD 3; C X3 C motif chemokine 1; C3Xkine; CX3CL1; CXC3; CXC3C; FKN; fractalkine; neurotactin; NTN; NTT; SCYD1; Small inducible cytokine D1

Expression system for standard: NS0; Immunogen sequence: Q25-R337

There is no detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC.

312pg/ml-20000pg/ml

CX3CL1

chemokine (C-X3-C motif) ligand 1

A0N0N7  

Mouse Fractalkine/CX3CL1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0357)

Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.

Catalog number	Description	Quantity
EK0357-CAP	96-well plate precoated with anti- mouse Fractalkine antibody	1
EK0357-ST	Lyophilized recombinant mouse Fractalkine standard	20ng/tube×2
EK0357-DA	Biotinylated anti- mouse Fractalkine antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	378	2168	9077	392	1975	9206
Standard deviation	17.76	101.89	653.54	23.52	114.55	810.12
CV(%)	4.7%	4.7%	7.2%	6%	5.8%	8.8%

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.157	0.229	0.371	0.444	0.718	1.199	1.913	2.184

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

3.5h